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Figure 4 | BMC Cancer

Figure 4

From: Up-regulation and subcellular localization of hnRNP A2/B1 in the development of hepatocellular carcinoma

Figure 4

The over-expression of hnRNP A2/B1 and hnRNP B1 in the mRNA and protein levels in HCC cell lines. (A) The mRNA expression of hnRNP A2/B1 in rat hepatocytes and rat HCC cell lines RH-35 and CBRH-7919. hnRNP A2/B1 mRNA product (450 bp) and β-actin mRNA product (230 bp) are shown in the figure. The blot presents two groups of total 6 independent RT-PCR experiments. The value in the graph is presented as mean ± standard deviation. (B) The mRNA expression of hnRNP B1 in rat hepatocytes and rat HCC cell lines RH-35 and CBRH-7919. hnRNP B1 mRNA product (900 bp) and β-actin mRNA product (230 bp) are shown in the figure. The blot presents two groups of total 6 independent RT-PCR experiments. The value in the graph is presented as mean ± standard deviation. (C) The protein expression of hnRNP A2/B1 in rat hepatocytes and two rat HCC cell lines. Equal amounts (15 μg) of whole cell protein extracts from rat hepatocytes, rat HCC cell RH-35 and rat HCC cell CBRH-7919 were loaded onto the SDS-PAGE gel and normalized by comparing with ß-actin. Western blot detected two protein bands of 35 kDa (hnRNP A2) and 37 kDa (hnRNP B1) in the whole cell extracts of rat HCC cell lines RH-35 and CBRH-7919. Western blot result is a representative of three independent experiments.

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