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Table 1 miR-183 and PDCD4 mRNA Expression Profiles in human hepatoma carcinoma tissues

From: miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells

Patient No.

Age

Gender

Tumer Size (cm*cm*cm)

Edmondson Grade

HBsAg

HCV-Ab

Cirrhosis

Normalized miR-183 Amount in Tumor Tissue Relative to Adjacent Normal Tissue 2-ΔΔCt

Normalized PDCD4 mRNA Amount in Tumor Tissue Relative to Adjacent Normal Tissue 2-ΔΔCt

1

35

F

5*4*4

3

Positive

Negative

Yes

32.90

0.37

2

52

M

8.5*7*6

2

Positive

Negative

No

16.68

0.41

3

43

M

3.5*3*2

2

Positive

Negative

Yes

6.36

0.89

4

41

F

12*10*8

3

Positive

Negative

No

3.39

0.32

5

34

M

10*7*7

3

Positive

Negative

Yes

4.96

0.41

6

50

M

2.5*2.5*2

3

Positive

Negative

No

23.59

0.74

7

33

M

10*8*7

4

Positive

Negative

Yes

0.33

1.45

8

50

M

2.5*1.8*1.5

1

Negative

Negative

No

0.53

0.34

9

52

M

10.5*8*5

3

Positive

Negative

Yes

2.08

0.4

10

52

M

10.5*8*5

3

Positive

Negative

Yes

9.06

0.92

11

63

M

14*10*10

2

Positive

Negative

Yes

1.29

1.25

12

70

M

3.5*3.5*3

2

Positive

Negative

Yes

25.28

0.08

13

66

F

15*7*5

3

Positive

Negative

No

48.84

0.31

14

39

M

12*11*6

3

Positive

Negative

No

5.94

0.50

15

51

M

2.5*2*2

2

Positive

Negative

Yes

101.83

0.35

16

47

M

5*5*4

2

Positive

Negative

Yes

0.28

0.98

17

37

M

16*4*8

2

Positive

Negative

Yes

367.09

0.21

18

52

M

8*7*4.5

2

Positive

Negative

No

0.39

1.57

19

64

M

8*5*4

2

Negative

Positive

No

0.17

6.19

20

50

F

6*5.5*2.5

1

Negative

Negative

No

130.69

0.2

21

52

M

7*7*7

3

Positive

Negative

Yes

1.38

0.23

22

56

M

8*6.5*5

3

Positive

Negative

Yes

0.88

0.47

23

48

M

2*2*1.5

2

Positive

Negative

No

48.17

0.17

24

59

M

8.8*8.5*8

2

Negative

Negative

No

41.93

0.26

25

40

M

2.5*2.5*2

2

Positive

Negative

Yes

16.34

0.41

  1. HBsAg indicates hepatitis B surface antigen; HCV-Ab, hepatitis C virus antibody; M, male; F, female; miRNA, microRNA. Relative quantification was performed by the 2-ΔΔCt method with adjacent normal liver tissue sample as a calibrator. Data show the means from three independent analyzes. Every independent analyze was carried out after the RNA extraction step. ΔCT obtained from real-time PCR was subject to paired t test. The expression levels of miR-183 in tumor tissues were significantly higher than in adjacent normal tissues (P < 0.01, t = - 3.07), and the expression levels of PDCD4 mRNA in tumor tissues were significantly lower than in adjacent normal tissues (P < 0.01, t = 3.69)