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Table 1 miR-183 and PDCD4 mRNA Expression Profiles in human hepatoma carcinoma tissues

From: miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells

Patient No. Age Gender Tumer Size (cm*cm*cm) Edmondson Grade HBsAg HCV-Ab Cirrhosis Normalized miR-183 Amount in Tumor Tissue Relative to Adjacent Normal Tissue 2-ΔΔCt Normalized PDCD4 mRNA Amount in Tumor Tissue Relative to Adjacent Normal Tissue 2-ΔΔCt
1 35 F 5*4*4 3 Positive Negative Yes 32.90 0.37
2 52 M 8.5*7*6 2 Positive Negative No 16.68 0.41
3 43 M 3.5*3*2 2 Positive Negative Yes 6.36 0.89
4 41 F 12*10*8 3 Positive Negative No 3.39 0.32
5 34 M 10*7*7 3 Positive Negative Yes 4.96 0.41
6 50 M 2.5*2.5*2 3 Positive Negative No 23.59 0.74
7 33 M 10*8*7 4 Positive Negative Yes 0.33 1.45
8 50 M 2.5*1.8*1.5 1 Negative Negative No 0.53 0.34
9 52 M 10.5*8*5 3 Positive Negative Yes 2.08 0.4
10 52 M 10.5*8*5 3 Positive Negative Yes 9.06 0.92
11 63 M 14*10*10 2 Positive Negative Yes 1.29 1.25
12 70 M 3.5*3.5*3 2 Positive Negative Yes 25.28 0.08
13 66 F 15*7*5 3 Positive Negative No 48.84 0.31
14 39 M 12*11*6 3 Positive Negative No 5.94 0.50
15 51 M 2.5*2*2 2 Positive Negative Yes 101.83 0.35
16 47 M 5*5*4 2 Positive Negative Yes 0.28 0.98
17 37 M 16*4*8 2 Positive Negative Yes 367.09 0.21
18 52 M 8*7*4.5 2 Positive Negative No 0.39 1.57
19 64 M 8*5*4 2 Negative Positive No 0.17 6.19
20 50 F 6*5.5*2.5 1 Negative Negative No 130.69 0.2
21 52 M 7*7*7 3 Positive Negative Yes 1.38 0.23
22 56 M 8*6.5*5 3 Positive Negative Yes 0.88 0.47
23 48 M 2*2*1.5 2 Positive Negative No 48.17 0.17
24 59 M 8.8*8.5*8 2 Negative Negative No 41.93 0.26
25 40 M 2.5*2.5*2 2 Positive Negative Yes 16.34 0.41
  1. HBsAg indicates hepatitis B surface antigen; HCV-Ab, hepatitis C virus antibody; M, male; F, female; miRNA, microRNA. Relative quantification was performed by the 2-ΔΔCt method with adjacent normal liver tissue sample as a calibrator. Data show the means from three independent analyzes. Every independent analyze was carried out after the RNA extraction step. ΔCT obtained from real-time PCR was subject to paired t test. The expression levels of miR-183 in tumor tissues were significantly higher than in adjacent normal tissues (P < 0.01, t = - 3.07), and the expression levels of PDCD4 mRNA in tumor tissues were significantly lower than in adjacent normal tissues (P < 0.01, t = 3.69)