Figure 3

miR-183 inhibits TGF-β1-induced PDCD4 expression in Huh7 cells. Cells were transfected with NC RNA, siPDCD4, or miR-183 respectively, after 24 hours, TGF-β1 (5 ng/mL) was added. Cells were harvested 24 hours later, and were subsequently processed for qRT-PCR (A) and Western blot (C) analysis by hybridization with antibodies against PDCD4 and β-actin. At the same time, the effect of TGF-β1 on miR-183 was examined (B). Huh7 cells were treated with TGF-β1 (5 ng/mL) for 48 h, then harvested and processed for qRT-PCR analysis.