Figure 4

Nfkb2 loss accelerates Myc-induced lymphomagenesis by impairing apoptosis. a) Effects of Nfkb2 deficiency on Myc-induced lymphomagenesis. The survival of Eμ-Myc transgenic littermates of the indicated Nfkb2 genotypes is shown. The differences in the rates of tumor incidence between the Nfkb2 ^+/+and the Nfkb2 ^-/-littermates is statistically significant (p = 0.0307). b) Eμ-Myc transgenic littermates of the indicated Nfkb2 genotypes were injected with BrdU, and cells from bone marrow and spleen were harvested 12 hr later. BrdU-incorporation was analyzed using an antibody-dependent fluorescence assay. The bars show the mean percentage of cells in S phase ± SEM (three independent experiments). c) The apoptotic index of the indicated genotypes was analyzed using an antibody dependent fluorescence assay. Annexin V⁺ B220⁺ cells of sIgM^- or sIgM⁺ phenotype are shown. The bars represent the mean ± SEM from three independent experiments. * indicates p < 0.05.