Figure 1
From: Neoplastic transformation of breast epithelial cells by genotoxic stress
Phenotypic characterization and cell cycle analyses of MCF 10A cells treated with radiation and cigarette smoke condensate. Panel A - Photomicrographs of MCF 10A-Rad + Csc treated cells (elongated) as compared to the parental MCF 10A cells (cuboidal). Panel B - Shows the percentage of cells distributed at the different phases of the cell cycle. Panel C - Immunoblot analyses of cell extracts from the control and treated MCF 10A cells. Primary antibodies were against p53 (Santa Cruz) and p21 (Cell Signaling). β-actin (Sigma) was scored for loading control.