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Table 1 Nomenclatures and sequences of PNAs

From: Modulation of mdm2 pre-mRNA splicing by 9-aminoacridine-PNA (peptide nucleic acid) conjugates targeting intron-exon junctions

Target (a) PNA No. Sequence (a)
5'-Int2 PNA2545 H-Acr-eg1- TCG GTG CTT ACC TGG-NH2
  PNA2547 H-Acr-eg1-TGC TTA CCT GGA TCA-NH2
  PNA2549 H-Acr-eg1-TTA CCT GGA TCA GCA-NH2
3'-Int2 PNA2551 H-Acr-eg1-TGT TGG TAT TGC ACA-NH2
  PNA2553 H-Acr-eg1-TGG TAT TGC ACA TTT-NH2
  PNA2555 H-Acr-eg1-TAT TGC ACA TTT GCC-NH2
  PNA2406 H-Acr-eg1-TGC ACA TTT GCC TAC-NH2
  PNA2557 H-Acr-eg1-ACA TTT GCC TAC AAG-NH2
  PNA2559 H-Acr-eg1-TTT GCC TAC AAG GAA-NH2
  PNA2561 H-Acr-eg1-GCC TAC AAG GAA AAA-NH2
3'-Int3 PNA2512 H-Acr-eg1-TTT GGT CTA ACC TAT -NH2
  PNA2967 H-(D-Arg)8-TTT GGT CTA ACC TAT-NH2
  PNA2968 H-(D-Arg)8-Lys(Deca)-TTT GGT CTA ACC TAT-NH2
PNA2406 mismatch PNA2681 H-Acr-eg1-TGC AGA TTT CCC TAC-NH2
PNA2512 mismatch PNA2733 H-Acr-eg1-TTT AGT CTA GCC TAT -NH2
  1. (a) Three PNAs are targeted to the 5'- splice site of intron2 of mdm2 pre-mRNA (5'-Int2). Seven PNAs are targeted to the 3'-splice site of intron2 (3'-Int2). PNA 2512 is targeted to the 3'- slice site of intron3 (3'-Int3). PNA2681 and PNA2733 are mismatch control PNAs for PNA2406 and PNA2512, respectively. Mismatches are indicated in bold. (b) The sequences of the PNAs (15-mer) are written from N-terminal to C-terminal-end. Acr and eg1 indicate 9-aminoacridinyl ligand and ethyleneglycol linker (8-amino-3,6-dioxaoctanoic acid), respectively.