Effect of PNA2512 on the protein level of MDM2 and p53. JAR cells were transfected with PNA 2512 or its mismatch PNA (PNA2733) and subjected to western blot analysis. Equal amount of protein were separated on SDS-PAGE and transferred to PVDF membrane. The membrane was probed with monoclonal antibody for MDM2 and reacting proteins were detected using the ECL-Plus system (Amersham). The membrane was washed and re-used for detection with a monoclonal antibody of p53 or α-tubulin. The numbers under the figure indicate the relative amount (normalized to α-tubulin) of the target proteins.