Figure 3

Expanded series of quantitative RT-PCRs in MM cells treated with different concentrations of Onconase for 24 h. Human MM cell lines M25, M29, M35, and M42 cells were treated with 0, 2.5, 5 and 10 μg/ml Onconase for 24 h, and RNA was isolated for real-time RT-PCR. Error bars represent standard deviation in the expression level of ATF3, IL-24, IL6, COX-2 and PTOV1 using different dilutions of total RNA. Results were validated in repeated experiments.