Figure 2
From: Anti-invasive and antiangiogenic effects of MMI-166 on malignant glioma cells
A new method for in vitro angiogenesis assay. The effects of MMI-166 on glioma-induced angiogenesis were determined by the newly devised research technique based on the angiogenesis kit (Kurabo, Osaka, Japan). Glioma cells were cultured in the insert plate so that soluble angiogenic factors secreted by the glioma cells could affect human umbical vein endothelial cells (HUVECs) in the lower chamber.