CS1 fibronectin enhanced SCC cell (HSC-3 and UM-SCC14A) migration and invasion in wound healing assays. (A) Repair of the wound by cell migration in the presence of a scrambled peptide (Scr1 and Scr2), CS1 peptide or CS1 blocking peptide was compared to that in control cells incubated in media alone at 0 and 16 h (Control, 0 and 16 h) and photographed (X200). (B and C) The total migrating distance of cells from edges of the wounds were measured after 16 h. (D) (Top left) Immunoblot showing the phospho FAK and FAK levels in controls or CS1 treated HSC-3 cells. (Top right) Immunoblot showing the FAK levels in HSC-3 cells after transfection with FAK antisense oligonucleotides (FAK-As) or scrambled sequence for 36 h (1.0 μg). (Middle and bottom panel), Cells were left untransfected or transfected with FAK antisense olgonucleotides (FAK-As) for 36 h. Repair of wound by cell migration with CS1 peptide or scramble peptide was photographed (X200). (E) The total migrating distance of cells from edges of the wounds (D middle and bottom panel) were measured after 16 h. (F) CS1 fibronectin mediates HSC-3 cell invasion. Fold change of cells invading through matrigel-coated pored membrane in control and CS1 treated group. Data represent mean ± SD from three independent experiments *, # p < 0.05.