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Figure 5 | BMC Cancer

Figure 5

From: Small RNA interference-mediated gene silencing of heparanase abolishes the invasion, metastasis and angiogenesis of gastric cancer cells

Figure 5

Silencing heparanase inhibited the in vitro angiogenesis of gastric cancer cells. Fifty microliters of growth factor-reduced matrigel was polymerized on 96-well plates. HUVECs were serum starved in RPMI1640 medium for 2 hrs, suspended in RPMI1640 medium preconditioned with siH3-transfected SGC-7901 cells, added to the matrigel-coated wells at the density of 5 × 104 cells/well, and incubated at 37°C for 18 hrs. Tube formation was visualized and calculated by measuring the length of tube walls formed between discrete endothelial cells in each well. Extensive tube formation of endothelial cells was observed in non-transfection (No) and scrambled siRNA (mock, 100 nmol/L) groups. However, when the endothelial cells were treated by the medium preconditioned with siH3-transfected SGC-7901 cells, the tube formation was dose-dependently suppressed. The symbols (* and **) indicates a significant (P < 0.05) and a very significant (P < 0.01) decrease from mock, respectively. Triplicate experiments were performed with essentially identical results.

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