Figure 3

Silencing heparanase attenuated the in vitro cell proliferation of gastric cancer cells. Confluent SGC-7901 cells were seeded into 96-well plates, and transfected with different concentrations (10 nmol/L, 50 nmol/L, and 100 nmol/L) of siH3 or scrambled siRNA (mock, 100 nmol/L). The untransfected cells served as a control (No). (A) Forty-eight hours post-transfection, MTT colorimetry was performed to assay the cell proliferation. The results indicated that high concentrations of siH3 (50 nmo/L and 100 nmol/L), but not low concentration of siH3 (10 nmol/L) or scrambled siRNA (mock, 100 nmol/L), attenuated the proliferation of SGC-7901 cells, when compared to the parental cells. (B) In colony formation assay, 48 hrs post-transfection, the cells were seeded at a density of 300/ml on 35-mm dishes. Colonies were allowed to grow for 10-14 days. Positive colony formation (more than 50 cells/colony) was counted. The results indicated that high concentrations of siH3 (50 nmo/L and 100 nmol/L), but not low concentration of siH3 (10 nmol/L) or scrambled siRNA (mock, 100 nmol/L), attenuated the cellular colony formation rates of cultured SGC-7901 cells. The symbols (* and **) indicates a significant (P < 0.05) and a very significant (P < 0.01) decrease from mock, respectively. Triplicate experiments were performed with essentially identical results.