Figure 4

Iripallidal effects expression of cell cycle regulators and DNA damage repair response molecules. Representative blot is shown from three independent experiments with identical results. C and I denote control and Iripallidal, respectively. Western blot analysis reveals (a) increase in p21, p27 and decrease in cyclin D1, cMyc levels (b) increase in γH2AX in A172, LN229, T98G and U87MG glioma cells treated with 20 μM Iripallidal, as compared to control. Figures (a) and (b) are representative blot shown from three independent experiments with identical results. Blots were reprobed for β-actin to establish equivalent loading.