Figure 6
Induction of apoptosis in T1 cells treated with poly(I:C) combined with a smac-mimetic (RMT 5265). A) Representation of the RMT 5265 molecule backbone drawn with MARVIN online software: http://atchimiebiologie.free.fr/marvin/doc/dev/oli.html. Substitution of one H on both alanines (R = CO2Bu-t) results in an inactive compound used as a negative control (HS4044). B) Flow cytometry assessment of the sub-G1 cell fraction in the T1 cell line treated with control compound or RMT 5265 (100 nM; 48 h) with or without poly(I:C)(500 ng/ml; last 16 h).