Figure 5

Enhanced apoptosis in Skov3 ovarian carcinoma cells treated with poly(I:C) combined with knocking-down c-IAP2 or XIAP. Skov3 cells were pre-treated with non-specific (NS), c-IAP2 or XIAP siRNAs with or without subsequent addition of poly(I:C). Cell protein extracts were subjected to western blot and stained successively for c-IAP2, XIAP, PARP and tubulin-α. PARP-cleavage was substantially enhanced by combination of poly (I:C) with c-IAP2 and XIAP siRNAs.