Figure 1

Assessment of TLR 3, 4 and 9 messenger RNAs and TLR3 protein in a panel of human cell lines in basal conditions and under stimulation by poly(I:C). A) Relative expression levels of TLR3, 4 and 9 transcripts in basal conditions. Levels of mRNAs in HeLa cells were chosen as a reference and arbitrarily set at 1. B) TLR mRNA relative increase in expression under stimulation by poly(I:C) (500 ng/ml) for 16 h. For each cell type, the basal level of expression was taken as a reference and set at 1. C) Western blot detection of the TLR3 protein in HeLa cells treated for 16 h with increasing concentrations of poly(I:C)(from 250 ng/ml to 2 μg/ml). β-actin staining was used to control protein loading. Data presented in A), B) and C) are representative of at least two similar experiments. The stars indicate a statistical difference from respective controls (p < 0.05).