Figure 5

HB-19 treatment of TIII cells results in the reduction of transcripts coding MMP-2, MMP-9 and TNF-α. A. Expression of transcripts 24 hours after HB-19 treatment. Two days after seeding, TIII cells were cultured for 24 hours in the absence (Control) or presence of 10 or 25 μM of HB-19. B. Expression of transcripts in control and HB-19 cultured TIII cells (cultured as described in Figure 3). C. Expression of transcripts in control and HB-19 precultured TIII cells (as in section B) that were further passaged 7-times in the absence of HB-19. The expression of specific mRNAs was investigated by RT-PCR using primers for MMP-2, MMP-9, VEGF-A, TNF-α, STAT-1, MIA and GAPDH.