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Figure 9 | BMC Cancer

Figure 9

From: Activation of endogenous p53 by combined p19Arf gene transfer and nutlin-3 drug treatment modalities in the murine cell lines B16 and C6

Figure 9

Animal model of B16 tumor formation reveals increased necrosis when cells were treated with both pCLPGp19 and nutlin-3. B16 cells were transduced ex vivo with pCLPG or pCLPGp19 followed by treatment with 25 ng/ml doxorubicin (dox) or 10 μM nutlin-3 (Nutlin) before subcutaneous implantation of 1 × 106 cells in C57BL/6 mice (n = 4/condition). After 15 days, the animals were sacrificed and tumors were analyzed histologically upon staining with HE. The ratio between necrotic and non-necrotic areas in photomicrographs of the HE sections was determined using ImageJ software and is presented as the mean and standard deviation of 15 to 20 fields from each of 3 sections from each tumor (*, student's t-test, p = 0.0012).

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