Figure 8From: Activation of endogenous p53 by combined p19Arf gene transfer and nutlin-3 drug treatment modalities in the murine cell lines B16 and C6 Volume of B16 tumors. B16 cells were transduced with either pCLPG (black bars) or pCLPGp19 (gray bars) and then treated with 25 ng/ml doxorubicin (Dox) or 10 μM nutlin-3, as indicated, before subcutaneous injection of 1 × 106 cells in C57BL/6 mice (n = 4/condition). After 15 days, the animals were sacrificed, tumors removed and measured. Tumor volume was determined using the formula V = π/6 × D1 × D2 × D3 (where D is the dimension in mm). Presented is the mean and standard deviation of the observed tumor volumes.Back to article page