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Figure 4 | BMC Cancer

Figure 4

From: Activation of endogenous p53 by combined p19Arf gene transfer and nutlin-3 drug treatment modalities in the murine cell lines B16 and C6

Figure 4

Western blot analysis of p19Arf and p21 (Cdkn1a) expression upon gene transfer and drug treatments. B16 (left) and C6 (right) cells were mock transduced or transduced with pCLPG or pCLPGp19 followed by treatment with 100 ng/doxorubicin or 10 μM nutlin-3 (lanes labeled D or N, respectively) or no drug (-). Exogenous p19Arf, endogenous p21 and β-Actin were revealed using specific primary antibodies followed by secondary HRP-conjugated antibody/ECL detection.

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