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Figure 3 | BMC Cancer

Figure 3

From: Activation of endogenous p53 by combined p19Arf gene transfer and nutlin-3 drug treatment modalities in the murine cell lines B16 and C6

Figure 3

Northern blot analysis reveals that presence of p19Arf aids pCLPG vector expression. B16 (A) or C6 cells (B) were mock transduced or transduced with equal quantities of pCLPG, pCLPGeGFP or pCLPGp19 in duplicate dishes. One dish from each duplicate was treated with 100 ng/ml of doxorubicin (Dox) and the other was maintained in fresh medium for 24 hours before harvesting total RNA used to perform the Northern blot analyses. Membranes were probed sequentially using the radiolabeled cDNAs of neomycin phosphotransferase (Neo), p19Arf (p19) or, as a control for loading, βActin.

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BMC Cancer

ISSN: 1471-2407

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