Table 1 Primer sequences, expected PCR product length and annealing temperature (temp) for growth factor receptor RT-PCR
From: Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
|  | Primer | Product (bp) | Temp (°C) |
|---|---|---|---|
EGFR | forward: 5'- ATG TCC GGG AAC ACA AAG AC -3' (2648-2667) | 351 | 54 |
NM_005228.3 | reverse: 5'- TTC CGT CAT ATG GCT TGG AT - 3' (2979-2998) | Â | Â |
IGFR1 | forward: 5'- ACC CGG AGT ACT TCA GCG CT -3' (2980-2999) | 230 | 50 |
NM_000875.3 | reverse: 5'-CAC AGA AGC TTC GTT GAG AA -3' (3190-3209) | Â | Â |
IGFR2 | forward: 5'- GCT GAC CAC TTG CTG TAG GAG AAG -3' (7162-7185) | 220 | 50 |
NM_000876.2 | reverse: 5'- ATC CTC ACT GTC CTG GTC ATC CC -3' (7359-7381) | Â | Â |
HGFR | forward: 5'- GGT CAA TTC AGC GAA GTC CT -3' (1481-1500) | 242 | 50 |
NM_000245.2 | reverse: 5'- TTC GTG ATC TTC TTC CCA GTG -3' (1702-1722) | Â | Â |
VEGFR1 | forward: 5'- TCG TGT AAG GAG TGG ACC ATC A -3' (1218-1239) | 470 | 50 |
NM_002019.3 | reverse: 5'- GCC AGA ACC ACT TGA TTG TAG G -3' (1690-1669) | Â | Â |
VEGFR2 | forward: 5'- GCG GTG ATT GCC ATG TTC TTC -3' (2616-2636) | 550 | 50 |
NM_002253.2 | reverse: 5'- CGC CGT TTC AGA TCC ACA GG -3' (3187-3168) | Â | Â |
VEGFR3 | forward: 5'- GGC AGC ATG GAG ATC GTG AT -3' (2393-2412) | 380 | 50 |
NM_182925.4 | reverse: 5'- GGT TGC CGA TGT GAA TGA GG -3' (2795-2776) | Â | Â |