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Table 2 Primers used for real-time quantitative RT-PCR evaluation of RNA of RANKL, OPG, BSP, OPN and β-actin.

From: The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts

Transcript name

 

RT-PCR primer set (5'-3')

Product length

β-actin

Forward

Reverse

ctggcacccagcacaatg

ccgatccacacggagtacttg

68

BSP

Forward

Reverse

tacacgggcgtcaatgaata

aggttccccgttctcacttt

63

OPG

Forward

Reverse

ggcaacacagctcacaagaa

cgctgttttcacagaggtca

117

OPN

Forward

Reverse

ttgcagtgatttgcttttgc

gccacagcatctgggtattt

115

RANKL

Forward

Reverse

agagcgcagatggatcctaa

ttccttttgcacagctcctt

180