Skip to main content

Table 1 Primers used for semi-quantitative RT-PCR evaluation of RNA of osteoblast-related genes and housekeeping gene.

From: The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts

Transcript name

 

RT-PCR primer set (5'-3')

Product length

β-actin

Forward

Reverse

tgacggggtcaccacactgtgcccatcta

ctagaagcatttgcggtggacgatggaggg

610

ALP

Forward

Reverse

cagaagctcaacaccaacg

ccagcaagaagaagcctttg

815

BMP2

Forward

Reverse

cccacttggaggagaaacaa

acgtctgaacaatggcatga

351

BSP

Forward

Reverse

atggcctgtgctttctcaat

ccgtttatgccttgttcgtt

497

Runx2

Forward

Reverse

aagaaggacagacagaagc

aggtggcagtgtcatcatct

428

COL-I

Forward

Reverse

agtgctagacatgctcagct

ttcaccatctctgcctgcgg

173

OPG

Forward

Reverse

tgcagtacgtcaagcaggag

gtgtcttggtcgccattttt

754

OPN

Forward

Reverse

ctaggcatcacctgtgccatacc

cagtgaccagttcatcagattcatc

395

OSN

Forward

Reverse

gtgcagaggaaaccgaagag

tcattgctgcacaccttctc

172

OSX

Forward

Reverse

ggcacaaagaagccgtactc

gccttgtaccaggagccata

296

PTHr

Forward

Reverse

aggaacagatcttcctgctgca

tgcatgtggatgtagttgcgcgt

521

RANKL

Forward

Reverse

agagcgcagatggatcctaa

ttccttttgcacagctcctt

180