Figure 7

Quantitative evaluation of expression of osteoblast-related genes in dasatinib-treated MSC. Cells were treated or not with 10^-8 M dasatinib and DAG, alone or in combination. RNA was isolated from MSC and subjected to real-time quantitative PCR using specific primers for osteoblast gene markers (RANKL, OPG, BSP, OPN) and β-actin (see Table 2). Data are presented as mean ± SEM from 4 independent experiments performed in duplicate; * p < 0.05, ** p < 0.01 and *** p < 0.001 versus the corresponding control, and comparing DAG versus Dasatinib+DAG.