Figure 5
Effect of dasatinib or E804 on Src phosphorylation. A: Cells treated for 30 min or 24 hours with 10-8, 10-7 M dasatinib or vehicle (Control) in complete culture medium. B: Cells exposed to 10-7 M dasatinib and/or DAG or vehicle for 24 hours. C: Cells stimulated for 24 hours with 10-7 M E804 or vehicle. A, B, C: Proteins (30 μg) were resolved by 10% SDS-PAGE and blotted onto nitrocellulose membranes. Immunodetections were performed using p-Src (Tyr 419) or Src antibodies, secondary antibodies and chemiluminescent substrate. Representative results from 2 independent experiments.