Table 1 MBD-mediated repression of CD44, Cyclin D2, GLIPR1 and PTEN
Repression (%) | Gal4-linked promoter Gal4BD-TRDs | methylated promoter full length proteins | unmethylated promoter full length proteins | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | MBD1 | MBD2 | MeCP2 | MBD1v1 | MBD1v3 | MBD2a | MBD2b | MeCP2 | MBD1v1 | MBD1v3 |
CD44 | MCF-7 | 55 | - | 20 | 85 | 5 | - | - | 45 | 75 | - |
| Â | DU145 | 75 | - | 55 | 80 | 25 | - | - | 10 | 75 | 25 |
| Â | LNCaP | 30 | - | - | 35 | - | - | - | - | 70 | 20 |
Cyclin D2 | MCF-7 | 65 | 50 | 65 | 90 | 45 | 50 | 45 | 90 | 75 | 50 |
| Â | DU145 | 30 | - | 15 | 65 | 30 | 5 | - | 50 | 20 | - |
| Â | LNCaP | 15 | 25 | 20 | 45 | 50 | 40 | 40 | 65 | 30 | - |
GLIPR | MCF-7 | 20 | - | 20 | 50 | 10 | - | - | 80 | 40 | - |
| Â | DU145 | 85 | 45 | 85 | 35 | - | 60 | 45 | 65 | 50 | - |
| Â | LNCaP | 20 | - | - | 20 | 25 | - | - | - | 20 | - |
PTEN | MCF-7 | 30 | - | 15 | 95 | 10 | 45 | 20 | 75 | 75 | 25 |
| Â | DU145 | 80 | - | 85 | 75 | - | - | - | - | 50 | 25 |
| Â | LNCaP | 20 | 10 | 20 | 30 | 20 | 45 | 35 | 55 | 65 | - |