Chromatin immunoprecipitation using antibodies specific for MBD1, MBD2 and MeCP2. Quantitative real-time PCR analysis of the immunoprecipitated DNA (IP) derived from unstimulated (basal) and 5-aza-CdR-stimulated DU145, LNCaP and MCF-7 cells was performed using primer pairs specific for the promoter fragments of RPLP0 (A), CD44 (B), Cyclin D2 (C), GLIPR1 (D), and PTEN (E). All values obtained were normalized and referred to 100% of the input DNA. * Statistical significance of p < 0.05 according to the Fisher's exact test. ** Statistical significance of p < 0.05 according to the analysis of variance (ANOVA).