Figure 2

Luciferase activities of the co-transfected reporter plasmids containing the promoters of CD44, Cyclin D2, GLIPR1 or PTEN in MCF-7, DU145 and LNCaP cells. MCF-7, DU145 and LNCaP cells were transiently transfected with pGL2-Luciferase reporter plasmids containing promoter fragments of CD44 (A), Cyclin D2 (B), GLIPR1 (C) or PTEN (D) immediately downstream of five Gal4 binding motifs (left diagrams), or with methylated reporter plasmids containing the same promoters without the Gal4 motifs (right diagrams). The co-transfected expression plasmids encoded either for Gal4-fused TRDs of MBD1, MBD2, or MeCP2 (left diagrams) or for full length proteins of MBD1v1, MBD1v3, MBD2a, MBD2b or MeCP2 proteins (right diagrams). The activities derived from the reference Renilla Luciferase were used for normalization of the data. The relative luciferase activities of the reporter constructs co-transfected with the empty expression plasmids (-) were arbitrarily set to 100%. * Statistical significance of p < 0.05 according to the Fisher's exact test in respect of repression of promoters by MBDs compared to the basal level activity (-).