Table 2 Technical data of the laboratory procedure for each specific antibody
Antibody | Antibody dilution | Manufacturer | Catalogue number | Type of antibody | Time of incubation (RT) | Pre-treatment buffer | visualization system |
|---|---|---|---|---|---|---|---|
AdipoR1 | 1:300 | Phoenix Pharmaceuticals | H-001-44 | Rabbit polyclonal | 30 min | Borgs decloaker | LSAB |
Adora1 | 1:500 | Genway Biotech | 18-461-10001 | Rabbit polyclonal | 30 min | Borgs decloaker | LSAB |
BTG2 | 1:1000 | Genway Biotech | 18-003-42396 | Rabbit polyclonal | 30 min | S2367 | Envision |
CD46 | 1:40 | BD Biosciences | 555948 | Mouse monoclonal | 30 min | S1699 | Envision |