Figure 3
Exosomes alter the morphology of invasive breast cancer cells. Immunofluorescence and cell morphological analysis of MDA-MB231 cells. (a) Cells were treated with PBS (top panel), exosomes isolated from MDA-MB231 cells (middle panel) or recombinant hsp90α protein (bottom panel) and stained with phalloidin to visualize F actin. Images taken at 10× magnification from three independent experiments. Scale bar = 100 μM. Inset: Representative images at 20× magnification. (b) Chang plot showing cell shape measurements. Cell shape is defined using the equation (4 × cell area)/cell perimeter2, where greater than 1 indicates a perfect circle and smaller values indicate a more irregular shape. Cell shape was calculated using OpenLab software. n = 130. (c) Cell area analysis of MDA-MB231 cells treated with exogenous exosomes or recombinant hsp90α. Cell area was calculated using OpenLab software. n = 130.