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Figure 4 | BMC Cancer

Figure 4

From: Inoculated mammary carcinoma-associated fibroblasts: contribution to hormone independent tumor growth

Figure 4

Cryostat sections of isolated EPI-HI or admixed with CAF-HI-GFP growing in BALB/c mice. (Upper panel) EPI-HI alone or admixed with CAF-HI-GFP were sc inoculated and mice were sacrificed at 4 or 13 days post inoculation. Before sacrifice, animals were perfused with 4% PFA. Cryostat sections were prepared, nuclei were counterstained with PI, and sections were visualized in a confocal microscope. Original magnification: 100× (Day 4) Bar: 30 μm; 200× (Day 13) Bar: 60 μm. (Lower panel) Immunofluorescence for SMA on day 13 after injection for EPI-HI and EPI-HI+CAF-HI tumors. Nuclei were counterstained with PI, and the sections were visualized in a confocal microscope. Original magnification: 200× (Day 13) Bar: 60 μm.

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