Cryostat sections of isolated EPI-HI or admixed with CAF-HI growing in BALB/c-GFP mice. (Upper panel) EPI-HI alone or admixed with CAF-HI were sc inoculated and animals were sacrificed at 13 or 28 days post inoculation. Before sacrifice, animals were perfused with 4% PFA. Cryostat sections were prepared; nuclei were counterstained with PI and sections visualized in a confocal microscope. Original magnification: 200×. Bar: 60 μm. (Lower panel) Immunofluorescence for SMA of primary cultures of EPI-HI and EPI-HI+CAF-HI tumors on day 13 after inoculation. Cells were seeded in chamber slides, fixed with 10% formalin and immunofluorescence was performed as described in Materials and Methods. Nuclei were counterstained with PI and slides were visualized in a confocal microscope. Original magnification: 400×. Bar: 60 μm.