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Figure 4 | BMC Cancer

Figure 4

From: Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis

Figure 4

Expression of galectin-7 is up-regulated in ESCC tissues relative to NEET tissues. (A) The magnified comparison maps, quantified 3D views and statistical analyses of galectin-7 expression in NEET and ESCC samples. (B) Representative samples of ESCC and NEET were resolved on 10% polyacrylamide gels and immunoblotted with an anti-galectin-7 antibody and a β-actin antibody as a loading control. The intensity of each band was measured with GraphPad Prism 4.0 software, and relative galectin-7 protein levels between ESCC and NEET samples were normalized against those of β-actin. The expression of galectin-7 was increased in tumor tissues. The difference in galectin-7 expression between ESCC and NEET samples was assessed using Student's t-test (*P < 0.05) for unpaired values. (C) Immunohistochemical analysis of galectin-7 expression in ESCC. Galectin-7 displayed positive staining in ESCC samples that was much stronger than the staining in non-neoplastic tissue. In normal esophageal epithelial tissues, galectin-7 is localized primarily to the nucleus. Expression of galectin-7 was detected ubiquitously in the cytoplasm, nuclei and membranes of ESCC cells. Images in the left panel were taken under 200× magnification; the right panel images are 400× magnifications of the same sections depicted on the left.

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