Figure 3

Growth of LNCaP-19 clones in vivo and in vitro. LNCaP-19 cells were implanted subcutaneously in nude mice and the experiment was ended when tumor size reached about 1.3 cm³, or after a maximum of 15 weeks. LNCaP-19 wild-type (L19 wt, n = 6), control transfected LNCaP-19 (L19C, n = 8) and ADAMTS1 transfected LNCaP-19 (L19A+, n = 8) (A) Tumor take rate is presented as percentage of animals with a palpable tumor at different time points after implantation. Fisher's exact test was used to compare the tumor take rate between groups, ** = P < 0.01, * = P < 0.05 L19A+ vs. L19C. (B) Tumor growth rate of L19C and L19A+ in castrated as well as in intact mice (intact mice; L19C, n = 8 and L19A+, n = 7) was calculated as tumor weight (mg) divided with the number of days from implantation to sacrifice and presented as the relative growth rate compared to wild-type tumors. Results are presented as mean ± SEM. Mann Whitney U test was used to compare differences between groups. (C) Tumor volumes were calculated as (length × width²)/2. (D) Proliferation rate in vitro of L19C and L19A+ in relation to L19 wt. Cells were seeded in 96-well plates in their regular cell culture medium. Four days later, DNA was quantified by a fluorescent dye and values were related to the wild-type cells. Mean ± SEM of two independent experiments with triplicates are shown.