Figure 1
From: ADAMTS1 alters blood vessel morphology and TSP1 levels in LNCaP and LNCaP-19 prostate tumors
Expression of ADAMTS1 in tumor xenografts. (A, D) mRNA expression of ADAMTS1 in tumor xenografts was analyzed by real-time RT-PCR, normalized to the expression of 18S rRNA and presented as relative expression compared to wild-type tumors. (B, E) Protein levels of ADAMTS1 in tumor xenografts were analyzed by western blotting. ADAMTS1 was detected in its latent form (110 kDa, arrows) as well as in its active form (87 kDa, arrowheads) and actin was used as loading control. Western blotting membranes showing one representative sample from each group. (C, F) Densitometric analysis presented as relative levels of active ADAMTS1 (87 kDa) to actin. Results are presented as mean ± SEM. Mann Whitney U test was used to compare differences between groups, ** = P < 0.01. (A-C) LNCaP wild-type (L wt, n = 8), control transfected LNCaP (LC, n = 8) and ADAMTS1 shRNA-transfected LNCaP (LA-, n = 6). (D-F) LNCaP-19 wild-type (L19 wt, n = 6), control transfected LNCaP-19 (L19C, n = 8) and ADAMTS1 transfected LNCaP-19 (L19A+, n = 6).