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Figure 7 | BMC Cancer

Figure 7

From: Characterization of AKT independent effects of the synthetic AKT inhibitors SH-5 and SH-6 using an integrated approach combining transcriptomic profiling and signaling pathway perturbations

Figure 7

Induction of mitotic defects by the specific PLC inhibitor U73122. Fixed SW480 cells preincubated with the specific PLC inhibitor U73122 for 48 hours were stained with anti-PRC1 (red), anti-γ-Tubulin (green) and DAPI (blue). We selected typical cells in different stages of the mitosis based on the DAPI staining: (A) Cells in metaphase with defect metaphase plate, (B) Cells in anaphase with failure in chromosome segregation, (C) Cells in anaphase with chromosome bridge, (D) Cells in telophase, daughter cell nuclei have different size, indicating failure in chromosome segregation (the white arrow marks the midbody between the daughter cells).

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