Skip to main content
Figure 6 | BMC Cancer

Figure 6

From: Characterization of AKT independent effects of the synthetic AKT inhibitors SH-5 and SH-6 using an integrated approach combining transcriptomic profiling and signaling pathway perturbations

Figure 6

Time lapse recording revealed failures in abscission of PIA-treated SW480 cells. Time lapse recording was started 24 hours after the incubation of SW480 cells with either SH-6 or DMSO as a vehicle control. Images were made every 30 seconds and aligned to movies. The bright field images token out of the time lapse movies show dividing SW480 cells in the time from 24 to 48 hours after treatment. Control (A) as well as SH-6 treated (B - D) cells progress similar through mitosis until cytokinesis. The daughter cells of the control samples will be separated during the cytokinesis, resulting in two individual cells (A). In contrast, the SH-6 treated cells fail to separate the bridge between the daughter cells, resulting in re-fusion and forming of binucleated cells (B - D). The time at the image corners is indicated in minutes.

Back to article page