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Table 1 Conditions for 5-Aza-dC and VPA treatments

From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines

5-Aza-dC treatment

  

   Experiment

5-Aza-dC (μM)

time (days)

cell proliferation

0.01, 0.1, 1, 10, 100

3

gene expression

4

1

methylation study

4

1

ChIP assay

4

1

soft agar assay

0.5, 2.5, 10

2

soft agar assay

0.1, 1

7

morphology

0.1

14, 21

VPA treatment

  

   Experiment

VPA (mM)

time (days)

ChIP assay

5

1