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Figure 6 | BMC Cancer

Figure 6

From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines

Figure 6

Effect of 5-Aza-dC on NB growth and tumorigenicity in vitro. A, In vitro cell proliferation assay demonstrating dose-response effects of 5-Aza-dC on LA1-55n cells. Each point represents the mean of four replicate experiments. B, Morphological alteration after treatment with 5-Aza-dC. LA1-55n cells were treated with 0.1 μM of 5-Aza-dC for 14 and 21 days respectively. Top photos (a and b) show the morphology of nontreated LA1-55n cells. Middle photos (c and d) show the morphology of LA1-55n cells treated with 5-Aza-dC for 14 days. Bottom photos (e and f) show the morphology of LA1-55n cells treated with 5-Aza-dC for 21 days. Green arrows indicated the flat "S" type cells. C, Quantitative analysis of percentage of N- type cells after treatment with 5-Aza-dC for 14 and 21 days. D, Quantitative analysis of colony formation on soft agar for 5-Aza-dC treated and untreated NB cells. Colonies were counted under a microscope. The average number of colonies was calculated and is shown as a percentage of the average number of colonies in the untreated group per cell line. Error bars represent standard deviation of colony numbers between different plates for each group.

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