Figure 4
From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines
Histone code map of THBS-1 promoter region. ChIP assay was performed on tumorigenic LA1-55n and non-tumorigenic LA1-5s cells. A, Schematic of the THBS-1 promoter. The vertical lines represent the location of CpG dinucleotide, and the orange line indicates the CpG island of the THBS-1 promoter region. The horizontal bars below the schematic indicate the location of the DNA fragments amplified by Q-PCR done on DNA recovered from ChIP experiments. B, C, and D, The enrichment of THBS-1 promoter DNA immunoprecipitated with antibodies specific for acetylated H3, acetyl H4, and H3K4Me3, respectively in LA1-5s cells. E, F, and G, The enrichment of THBS-1 promoter DNA immunoprecipitated with antibodies specific for H3K9Me3, H3K27Me3, and H3K27Me2, respectively in LA1-55n cells.