Figure 2
From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines
Alteration of DNA methylation degree after treatment with 5-Aza-dC. Genomic DNA from LA1-55n, LA1-5s, and LA1-55n treated with 4 μM 5-Aza-dC was isolated and modified by sodium bisulfite. Quantitative methylation-specific PCR was performed to examine the methylation degree around promoter regions for four cancer related genes (THBS-1, HIN-1, TIG-1, and CASP8) in LA1-55n, LA1-5s as well as LA1-55n cells treated with 5-Aza-dC.