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Figure 3 | BMC Cancer

Figure 3

From: Epigallocatechin-3-gallate suppresses the expression of HSP70 and HSP90 and exhibits anti-tumor activity in vitro and in vivo

Figure 3

FEGCG suppresses the expression of HSP70 and HSP90 in MCF-7 cells. (A) The cells were treated with EGCG for 24 h and the expression levels of heat shock proteins were determined by Western blot analysis. (B) The levels of HSP90, HSP70, and HSP60 mRNA were detected by RT-PCR analysis. GAPDH was used as an internal control. (C) The cells were harvested 24 h after EGCG treatment. Cell lysates were subjected to Western blot analysis for Akt and Bcl-2. (D), (E) MCF-7 cells were transfected with pGL3-HSP70 (D) or pXP2-HSP90 (E) reporter vector. After 24 h, cells were heat shocked at 42°C for 1 h followed by recovery at 37°C for 24 h with EGCG (100 μM). Cells were harvested and the cell extracts were subjected to luciferase assay. The results were represented as the mean ± SD of three independent experiments. Asterisk, P < 0.05, significantly different from control. (F) The cells were treated with indicated concentrations of EGCG for 24 h. The expression levels of HSF1 and HSF2 were monitored by Western blot analysis.

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