Table 1 Primers and probes used for bisulfite sequencing and MSP analysis

hTERT S1/-442 to -219

F 5' GGGTTATTTTATAGTTTAGGT 3'

R 5' AATCCCCAATCCCTC 3'

hTERT S2/-208 to +104

F 5' GTTTTGTTTTTTTATTTTTTAGTTT 3'

R 5' CCAACCCTAAAACCCCAAA 3'

hTERT S3/+88 to +348

F 5' TTTGGGGTTTTAGGGTTGG-3'

R 5' AACCACCAACTCCTTCAAA-3'

hTERT S4/+319 to +566

F 5' GTAGGTGTTTTGTTTGAAGGA-3'

R 5' TACCAACAAATAAACCAAC-3'

MSP primers/

Location

Sequences*

hTERT M1/-317 to -262

F 5' TAGATTTTCGGGTTCGTTCG 3'

R 5' TCTATACCCGCGAATCCACT 3'

P 5' CGACCTAACCCCGACAACGCAACTA 3'

hTERT M2/+288 to +419

F 5' GAGTAGCGTAGGCGATTTAGGGCGT 3'

R 5' GTCCAACAACGCGAAACCGAA 3'

P 5' CGCACAACCTCTACAACACTCGAACCACCAACTC 3'

β actin

F 5' TGGTGATGGAGGAGGTTTAGTAAGT 3'

R 5' AACCAATAAAACCTACTCCTCCCTTAA 3'

P 5' ACCACCACCCAACACACAATAACAAACACA 3'