Figure 5

The miR-21 inhibitor enhanced taxol induced apoptosis. Flow cytometry analyses of propidium iodide-stained cells were performed in triplicate (Fig. 5A). Percentages of apoptotic cells are shown in the histogram (Fig. 5B). The effectiveness of the miR-21 inhibitor combined with taxol on BcL-2 and caspase-3 was analyzed by western blotting, with mock cells as controls (Fig. 5C). Antibodies against β-actin were used as a loading control to ensure uniform protein loading in all lanes.