Figure 2

Effect of the miR-21 inhibitor on the chemo-sensitivity of U251 and LN229 cells to taxol treatment. The growth of U251 (Figure 2a) and LN229 (Figure 2b) cells were inhibited by the miR-21 inhibitor, taxol only, and the indicated combinations. The cells were treated with the miR-21 inhibitor complexed to PAMAM for 6 h at 37°C. The medium was then replaced with media containing various concentrations of taxol. After 72 h of incubation, an MTT assay was performed. Absorbance at 570 nm was normalized to the control (untreated cells) to determine cell viability. Each value represents the mean ±SD from triplicate determinations. An aqueous solution of taxol (circles) and miR-21 inhibitor-loaded PAMAM (triangle) was incubated with human glioblastoma U251 (Figure 2c) and LN229 (Figure 2d) cells for six days. Drug-induced decrease in cell numbers was measured using the MTT assay. Values represent the mean ± SD (n = 6 replicates).