Effect of BITC and PEITC on L9981 cells invasion. Invasion Chamber Assays were performed to assess the effect on cell invasion. Cells were treated or untreated with 5 μM of BITC or 10 μM of PEITC for 24 h. (A) Representative photographs of treated and untreated cells are presented (×200 magnification). (B) Number of cells invaded at 24 h time point. Values represent the mean ± SD of three independent experiments (*** P < 0.001).