Figure 1
From: Methylthioadenosine (MTA) inhibits melanoma cell proliferation and in vivotumor growth
Methylthioadenosine inhibits melanoma cell growth. Proliferation assays of (A) 37-31E and MeWo (wild type RAS BRAF), (B) SKMel147 and SKMel103 (NRASQ61L mutated) and (C) UACC903 and Colo829 (BRAFV600E mutated) melanoma cells in complete medium with increasing concentrations of Methylthioadenosine (MTA). Medium was changed adding fresh MTA every 48 h. Proliferation assays were performed by counting the number of viable cells using Guava-Viacount reagent (Guava Technologies) in a cell counter (Viacount) at the time points indicated. Asterisk indicate p < 0.05. (D) Graph showing the percentage of cell proliferation inhibition at 96 h treated with 10 μM MTA. p-values were calculated performing a t-student test. (E) Western-blot showing the levels of MTAP in the different cell lines. The mutational status of CDK2NA for the different cell lines is showed in the table.