Table 1 List of primary antibodies used in immunohistochemical analyses.
Primary Antibody | Marker for: | Source | Dilution |
|---|---|---|---|
Mouse anti keratin 18 | Luminal epithelial cells | Sigma-Aldrich (St. Louis, MO) | 1:500 |
Rabbit anti Ki67 | Proliferation | Vector (Burlingame, CA) | 1:3000 |
Mouse anti E-cadherin | Cell-cell adhesion | Novocastra (Newcastle, UK) | 1:50 |
Mouse anti laminin 5 | Basement membrane | Chemicon/Millipore (Bedford, MA) | 1:200 |
Mouse anti sialomucin | Apical proteins | Abcam (Cambridge, MA) | 1:800 |
Mouse anti-CEACAM | Cell-cell adhesion | Abcam (Cambridge, MA) | 1:50 |