Figure 3

Histopathology and immunohistochemistry of platelet binding and PF-4 in prostates of the Lady mouse. Prostate glands from Lady mice receiving either standard diet or an E/S/L-supplemented diet were excised and processed for histomorphology and immunohistochemistry according to Materials and Methods. H&E staining reveals tumor burden with poorly differentiated carcinoma in prostates of mice receiving a standard diet, while a normal prostate architecture is evident in mice receiving an E/S/L-supplemented diet (Panel A, 20× magnification). Distinct intravascular staining for PF-4 is evident in prostates of Lady mice receiving E/S/L compared to that of the control group (red arrows, Panel B-20X and Panel C-40X magnification, respectively). No positive staining for PF-4 is evident in the lymphatics (blue arrows). Distinct intravascular staining for platelets is evident in prostates of Lady mice receiving the test diet compared to that of the control group (red arrows, Panel D-20X magnification). No platelet binding is evident in the lymphatics (blue arrows).